Regulatory
Part:BBa_K1059003:Design
Designed by: Qiu Wang Group: iGEM13_OUC-China (2013-09-17)
Its transcript can prevent the mRNA itself from being degraded by RNaseE.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To make sure the 5' ribonucleotide protected by a ribosome,we must arrange the RBS close to the 5' extreme ribonucleotide.And also ,the "extra RBS " and the "real RBS " must be enough interval, preventing the ribosome interfe with each other.
Source
This segment is design by oueselves.